Author: Pizzutto, Susan J.; Upham, John W.; Yerkovich, Stephanie T.; Chang, Anne B.
Title: High Pulmonary Levels of IL-6 and IL-1ß in Children with Chronic Suppurative Lung Disease Are Associated with Low Systemic IFN-? Production in Response to Non-Typeable Haemophilus influenzae Document date: 2015_6_12
ID: 19jo817j_16
Snippet: IFN-γ, IL-1β, IL-6, IL-8, IL-12 p70, LL-37and IP-10 in plasma and BAL, and IFN-γ and in PBMC culture supernatants were measured in our research laboratory using an in-house dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA) as described previously [7, 18] . Quantitative standard curves were generated from serial dilutions of recombinant human proteins and included on each plate. The limit of detection was 10 pg/ml. The cathelici.....
Document: IFN-γ, IL-1β, IL-6, IL-8, IL-12 p70, LL-37and IP-10 in plasma and BAL, and IFN-γ and in PBMC culture supernatants were measured in our research laboratory using an in-house dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA) as described previously [7, 18] . Quantitative standard curves were generated from serial dilutions of recombinant human proteins and included on each plate. The limit of detection was 10 pg/ml. The cathelicidin LL-37 was measured in plasma and BAL using a high sensitivity ELISA kit (Hycult Biotech, The Netherlands). The limit of detection was 0.14 ng/ml. For analysis purposes, a value of 1/10 the limit of detection was assigned if the marker concentration was below the limit of detection.
Search related documents:
Co phrase search for related documents- analysis purpose and human protein: 1
- DELFIA lanthanide fluorescent immunoassay and lanthanide fluorescent: 1
- DELFIA lanthanide fluorescent immunoassay and lanthanide fluorescent immunoassay: 1
- detection limit and ELISA kit: 1, 2, 3, 4, 5, 6
- detection limit and high sensitivity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- detection limit and human protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- ELISA kit and high sensitivity: 1, 2, 3, 4
- ELISA kit and human protein: 1, 2
- ELISA kit and marker concentration: 1
- high sensitivity and human protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
- high sensitivity and lanthanide fluorescent: 1
- high sensitivity and marker concentration: 1
- human protein and lanthanide fluorescent: 1
- human protein and previously describe: 1
Co phrase search for related documents, hyperlinks ordered by date