Author: Pizzutto, Susan J.; Upham, John W.; Yerkovich, Stephanie T.; Chang, Anne B.
Title: High Pulmonary Levels of IL-6 and IL-1ß in Children with Chronic Suppurative Lung Disease Are Associated with Low Systemic IFN-? Production in Response to Non-Typeable Haemophilus influenzae Document date: 2015_6_12
ID: 19jo817j_16
Snippet: IFN-γ, IL-1β, IL-6, IL-8, IL-12 p70, LL-37and IP-10 in plasma and BAL, and IFN-γ and in PBMC culture supernatants were measured in our research laboratory using an in-house dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA) as described previously [7, 18] . Quantitative standard curves were generated from serial dilutions of recombinant human proteins and included on each plate. The limit of detection was 10 pg/ml. The cathelici.....
Document: IFN-γ, IL-1β, IL-6, IL-8, IL-12 p70, LL-37and IP-10 in plasma and BAL, and IFN-γ and in PBMC culture supernatants were measured in our research laboratory using an in-house dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA) as described previously [7, 18] . Quantitative standard curves were generated from serial dilutions of recombinant human proteins and included on each plate. The limit of detection was 10 pg/ml. The cathelicidin LL-37 was measured in plasma and BAL using a high sensitivity ELISA kit (Hycult Biotech, The Netherlands). The limit of detection was 0.14 ng/ml. For analysis purposes, a value of 1/10 the limit of detection was assigned if the marker concentration was below the limit of detection.
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