Selected article for: "clinical potential and high sensitivity"
Author: Barzon, Luisa; Lavezzo, Enrico; Militello, Valentina; Toppo, Stefano; Palù, Giorgio
Title: Applications of Next-Generation Sequencing Technologies to Diagnostic Virology
  • Document date: 2011_11_14
    • ID: 01nuj0lk_42
    Snippet: Clonal sequencing of RT-PCR products by 454 pyrosequencing offers the advantage of high sensitivity for minor variants and a relatively long sequence length that facilitates the characterization of the linkage amongst resistance mutations and avoids the risk to miss mutations due to sequence variation around the site under investigation. The application of 454 sequencing-based resistance testing in clinical setting, however, requires careful cons.....
    Document: Clonal sequencing of RT-PCR products by 454 pyrosequencing offers the advantage of high sensitivity for minor variants and a relatively long sequence length that facilitates the characterization of the linkage amongst resistance mutations and avoids the risk to miss mutations due to sequence variation around the site under investigation. The application of 454 sequencing-based resistance testing in clinical setting, however, requires careful consideration of potential technical errors that can be introduced in the experimental protocol and in data analysis in order to discriminate between experimentally introduced errors and true variants [87, 100, 101] . Data analysis issues are discussed in Section 2.9.

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