Author: Chenoll, Empar; Casinos, Beatriz; Bataller, Esther; Buesa, Javier; Ramón, Daniel; Genovés, Salvador; Fábrega, Joan; Rivero Urgell, Montserrat; Moreno Muñoz, José A.
Title: Identification of a Peptide Produced by Bifidobacterium longum CECT 7210 with Antirotaviral Activity Document date: 2016_5_4
ID: 0sxl6f1r_29
Snippet: The influence of zinc, potassium, and calcium on the purified protease activity was studied. As above, the assay was carried out with β-casein (2% w/v) as substrate and 70 µL citratephosphate buffer (pH 6.4, 50 mM). Incubation conditions were 50 • C (optimum temperature) for 72 h. Minerals were added separately at a final concentration of 10 mM. After incubation, samples were boiled and 0.45 µm pore-size filtered, and 11mer was analyzed by H.....
Document: The influence of zinc, potassium, and calcium on the purified protease activity was studied. As above, the assay was carried out with β-casein (2% w/v) as substrate and 70 µL citratephosphate buffer (pH 6.4, 50 mM). Incubation conditions were 50 • C (optimum temperature) for 72 h. Minerals were added separately at a final concentration of 10 mM. After incubation, samples were boiled and 0.45 µm pore-size filtered, and 11mer was analyzed by HPLC. A control was included without the addition of any mineral.
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