Author: van der Schaar, H. M.; Melia, C. E.; van Bruggen, J. A. C.; Strating, J. R. P. M.; van Geenen, M. E. D.; Koster, A. J.; Bárcena, M.; van Kuppeveld, F. J. M.
Title: Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein Document date: 2016_7_6
ID: 1aptufp6_43
Snippet: Quantitative PCR. RNA was isolated from infected cells using a NucleoSpin RNA kit (Macherey-Nagel). cDNA was synthesized using random hexamers as primers with a TaqMan reverse transcription reagent kit (Roche). The cDNA was used for quantitative PCR with the forward primer 5=-CGTGGGGCT ACAATCAAGTT-3=, the reverse primer 5=-TAACAGGAGCTTTGGGCATC-3=, and the LightCycler 480 SYBR green I master kit (Roche) for 45 cycles (5 s at 95°C, 10 s at 60°C, .....
Document: Quantitative PCR. RNA was isolated from infected cells using a NucleoSpin RNA kit (Macherey-Nagel). cDNA was synthesized using random hexamers as primers with a TaqMan reverse transcription reagent kit (Roche). The cDNA was used for quantitative PCR with the forward primer 5=-CGTGGGGCT ACAATCAAGTT-3=, the reverse primer 5=-TAACAGGAGCTTTGGGCATC-3=, and the LightCycler 480 SYBR green I master kit (Roche) for 45 cycles (5 s at 95°C, 10 s at 60°C, and 20 s at 72°C) on a LightCycler 480 system (Roche).
Search related documents:
Co phrase search for related documents- cdna quantitative PCR and quantitative PCR: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21
- cdna quantitative PCR and reverse primer: 1, 2, 3
- cdna quantitative PCR and reverse primer forward primer: 1
- cdna quantitative PCR and RNA kit: 1, 2, 3, 4, 5, 6
- forward primer and infected cell: 1, 2, 3
- forward primer and master kit: 1
- forward primer and quantitative PCR: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
- forward primer and random hexamer: 1
- forward primer and reverse primer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- forward primer and reverse primer forward primer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- forward primer and RNA kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- infected cell and quantitative PCR: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- infected cell and reverse primer: 1, 2, 3
- infected cell and reverse primer forward primer: 1
- infected cell and RNA kit: 1, 2, 3, 4, 5, 6
- Macherey Nagel NucleoSpin RNA kit and master kit: 1
- Macherey Nagel NucleoSpin RNA kit and NucleoSpin RNA kit: 1, 2, 3, 4, 5, 6, 7, 8, 9
- Macherey Nagel NucleoSpin RNA kit and RNA kit: 1, 2, 3, 4, 5, 6, 7, 8, 9
- Macherey Nagel NucleoSpin RNA kit and Roche master kit: 1
Co phrase search for related documents, hyperlinks ordered by date