Author: van der Schaar, H. M.; Melia, C. E.; van Bruggen, J. A. C.; Strating, J. R. P. M.; van Geenen, M. E. D.; Koster, A. J.; Bárcena, M.; van Kuppeveld, F. J. M.
Title: Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein Document date: 2016_7_6
ID: 1aptufp6_43
Snippet: Quantitative PCR. RNA was isolated from infected cells using a NucleoSpin RNA kit (Macherey-Nagel). cDNA was synthesized using random hexamers as primers with a TaqMan reverse transcription reagent kit (Roche). The cDNA was used for quantitative PCR with the forward primer 5=-CGTGGGGCT ACAATCAAGTT-3=, the reverse primer 5=-TAACAGGAGCTTTGGGCATC-3=, and the LightCycler 480 SYBR green I master kit (Roche) for 45 cycles (5 s at 95°C, 10 s at 60°C, .....
Document: Quantitative PCR. RNA was isolated from infected cells using a NucleoSpin RNA kit (Macherey-Nagel). cDNA was synthesized using random hexamers as primers with a TaqMan reverse transcription reagent kit (Roche). The cDNA was used for quantitative PCR with the forward primer 5=-CGTGGGGCT ACAATCAAGTT-3=, the reverse primer 5=-TAACAGGAGCTTTGGGCATC-3=, and the LightCycler 480 SYBR green I master kit (Roche) for 45 cycles (5 s at 95°C, 10 s at 60°C, and 20 s at 72°C) on a LightCycler 480 system (Roche).
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