Selected article for: "forward primer and reverse primer forward primer"
Author: van der Schaar, H. M.; Melia, C. E.; van Bruggen, J. A. C.; Strating, J. R. P. M.; van Geenen, M. E. D.; Koster, A. J.; Bárcena, M.; van Kuppeveld, F. J. M.
Title: Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein
  • Document date: 2016_7_6
    • ID: 1aptufp6_43
    Snippet: Quantitative PCR. RNA was isolated from infected cells using a NucleoSpin RNA kit (Macherey-Nagel). cDNA was synthesized using random hexamers as primers with a TaqMan reverse transcription reagent kit (Roche). The cDNA was used for quantitative PCR with the forward primer 5=-CGTGGGGCT ACAATCAAGTT-3=, the reverse primer 5=-TAACAGGAGCTTTGGGCATC-3=, and the LightCycler 480 SYBR green I master kit (Roche) for 45 cycles (5 s at 95°C, 10 s at 60°C, .....
    Document: Quantitative PCR. RNA was isolated from infected cells using a NucleoSpin RNA kit (Macherey-Nagel). cDNA was synthesized using random hexamers as primers with a TaqMan reverse transcription reagent kit (Roche). The cDNA was used for quantitative PCR with the forward primer 5=-CGTGGGGCT ACAATCAAGTT-3=, the reverse primer 5=-TAACAGGAGCTTTGGGCATC-3=, and the LightCycler 480 SYBR green I master kit (Roche) for 45 cycles (5 s at 95°C, 10 s at 60°C, and 20 s at 72°C) on a LightCycler 480 system (Roche).

    Search related documents:
    Co phrase search for related documents
    • master kit and quantitative PCR: 1
    • master kit and random hexamer: 1
    • master kit and reverse primer: 1
    • master kit and reverse primer forward primer: 1
    • NucleoSpin RNA kit and quantitative PCR: 1
    • NucleoSpin RNA kit and RNA kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    • NucleoSpin RNA kit and Roche master kit: 1
    • quantitative PCR and random hexamer: 1, 2
    • quantitative PCR and reverse primer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
    • quantitative PCR and reverse primer forward primer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • quantitative PCR and RNA kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46
    • quantitative PCR and transcription reagent: 1, 2
    • quantitative PCR and transcription reagent kit: 1
    • random hexamer and reverse primer: 1, 2, 3, 4
    • random hexamer and reverse primer forward primer: 1
    • random hexamer and RNA kit: 1, 2, 3, 4, 5, 6
    • reverse primer and RNA kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
    • reverse primer forward primer and RNA kit: 1, 2, 3, 4, 5, 6, 7, 8
    • RNA kit and Roche master kit: 1, 2