Author: Izquierdo, Laure; Oliveira, Catarina; Fournier, Carole; Descamps, Véronique; Morel, Virginie; Dubuisson, Jean; Brochot, Etienne; Francois, Catherine; Castelain, Sandrine; Duverlie, Gilles; Helle, Francois
Title: Hepatitis C Virus Resistance to Carbohydrate-Binding Agents Document date: 2016_2_12
ID: 1a4l1beo_13
Snippet: HuH-7-RFP-NLS-IPS were infected with a stock of the HCV JFH1-CS-A4 strain (designated i6 stock in [17] ) in the presence of the 90% effective concentration of GNA, CV-N, ConA or GRFT (1, 0.1, 2 and 1 μg/mL, respectively). Infected cells were subcultured with administration of the lectins at the same concentration. When 100% cells were infected, the supernatants were recovered and used to infect naive cells in the presence of the lectins. The esc.....
Document: HuH-7-RFP-NLS-IPS were infected with a stock of the HCV JFH1-CS-A4 strain (designated i6 stock in [17] ) in the presence of the 90% effective concentration of GNA, CV-N, ConA or GRFT (1, 0.1, 2 and 1 μg/mL, respectively). Infected cells were subcultured with administration of the lectins at the same concentration. When 100% cells were infected, the supernatants were recovered and used to infect naive cells in the presence of the lectins. The escalation of lectin concentrations as well as the time points at which viruses were recovered and used to infect naive cells are depicted in Fig 1.
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