Author: Kummer, Susann; Avinoam, Ori; Kräusslich, Hans-Georg
Title: IFITM3 Clusters on Virus Containing Endosomes and Lysosomes Early in the Influenza A Infection of Human Airway Epithelial Cells Document date: 2019_6_12
ID: 1345qct4_17
Snippet: The 2D object counter and coloc2 plugin in Fiji software (https://fiji.sc/, 64-bit version)were used for the cluster and Mander's Coefficient Correlation (MCC) analyses, respectively. MCC was performed using the Costes regression threshold. The defined objects were assigned as a cluster being larger than 10 5 nm 2 based on STED images with approximately 50 nm resolution. All graphs show the mean values of three independent experiments plotted wit.....
Document: The 2D object counter and coloc2 plugin in Fiji software (https://fiji.sc/, 64-bit version)were used for the cluster and Mander's Coefficient Correlation (MCC) analyses, respectively. MCC was performed using the Costes regression threshold. The defined objects were assigned as a cluster being larger than 10 5 nm 2 based on STED images with approximately 50 nm resolution. All graphs show the mean values of three independent experiments plotted with the standard error calculated as a two tailed unpaired t-test. A statistical analysis was performed using GraphPad Prism software (version 5).
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