Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase

Takenouchi, Takato; Kitani, Hiroshi; Suzuki, Shunichi; Nakai, Michiko; Fuchimoto, Dai-ichiro; Tsukimoto, Mitsutoshi; Shinkai, Hiroki; Sato, Mitsuru; Uenishi, Hirohide

Furthermore, we assessed the capacity of the IPKM to produce inflammatory and anti-inflammatory cytokines in response to stimulation with LPS. As was found for the primary PKM, the IPKM secreted substantial amounts of inflammatory cytokines, such as tumor necrosis factor α and IL-1β, after being stimulated with LPS for 24 h (Figure 4 , upper and middle panels). In contrast, although the primary PKM secreted a substantial amount of the anti-inflammatory cytokine IL-10 upon LPS stimulation, the IPKM failed to secrete measurable amounts of IL-10 (Figure 4, lower panel) . These findings suggest that the IPKM primarily displayed the pro-inflammatory M1 phenotype in response to bacterial endotoxins (20) . Further studies using various stimuli are required to determine whether IPKM can exhibit the antiinflammatory M2 phenotype.