Selected article for: "copy number and dna copy"
Author: Jordana-Lluch, Elena; Giménez, Montserrat; Quesada, M. Dolores; Ausina, Vicente; Martró, Elisa
Title: Improving the Diagnosis of Bloodstream Infections: PCR Coupled with Mass Spectrometry
  • Document date: 2014_4_9
    • ID: 05zahl5n_21
    Snippet: Another feature of this technology is that it allows a relative quantification of the microorganism present in the specimen. This is achieved by the use of an internal standard that is amplified with the same primer pairs as those for amplification of the target gene. The internal standard has a different base composition and thus can be differentiated. As this synthetic standard is added to each PCR well at a known copy number, the comparison be.....
    Document: Another feature of this technology is that it allows a relative quantification of the microorganism present in the specimen. This is achieved by the use of an internal standard that is amplified with the same primer pairs as those for amplification of the target gene. The internal standard has a different base composition and thus can be differentiated. As this synthetic standard is added to each PCR well at a known copy number, the comparison between standard and microbial DNA permits quantification. In the absence of a PCR product, the internal standard serves as PCR positive control to exclude PCR inhibition.

    Search related documents:
    Co phrase search for related documents
    • base composition and positive control: 1
    • copy number and internal standard: 1
    • copy number and PCR inhibition: 1, 2
    • copy number and PCR positive control: 1
    • copy number and PCR product: 1, 2, 3, 4, 5, 6
    • copy number and positive control: 1, 2, 3, 4, 5, 6, 7
    • copy number and relative quantification: 1, 2
    • dna microbial standard and microbial standard: 1
    • internal standard and relative quantification: 1
    • microbial standard and positive control: 1
    • PCR add and positive control: 1
    • PCR add and primer pair: 1, 2
    • PCR inhibition and positive control: 1, 2, 3, 4, 5, 6
    • PCR positive control and positive control: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • PCR product and positive control: 1, 2, 3
    • PCR product and primer pair: 1, 2, 3, 4, 5, 6
    • PCR product and relative quantification: 1
    • positive control and primer pair: 1, 2
    • positive control and relative quantification: 1