Identification of Common Biological Pathways and Drug Targets Across Multiple Respiratory Viruses Based on Human Host Gene Expression Analysis

Smith, Steven B.; Dampier, William; Tozeren, Aydin; Brown, James R.; Magid-Slav, Michal

The QC analysis assessed each sample in the dataset for kernel density, PCA, MAD, and pair-wise Pearson correlation such that: 1) the kernel density was normally distributed; 2) after PCA values were within the Hotelling T2 alpha level threshold of 0.05 [91] [92] [93] ; 3) MAD score scores were in the range of +3 to 23 with no outliers [94] ; and 4) inner-treatment group pair wise correlations for samples derived from a single cell were $0.97 or $0.90 if taken from individual donors [94] . Figures were created using Array Studio software, version 4.1. (Omicsoft Corporation, Research Triangle Park, NC, USA [95] ). During subsequent analysis, each comparison group was treated separately, regardless of dataset origination, in order to gain a wider, less bias view of representative genes and pathways.