Author: Kummer, Susann; Avinoam, Ori; Kräusslich, Hans-Georg
Title: IFITM3 Clusters on Virus Containing Endosomes and Lysosomes Early in the Influenza A Infection of Human Airway Epithelial Cells Document date: 2019_6_12
ID: 1345qct4_16
Snippet: Fluorescence imaging was performed using a two-colour-STED microscopy instrumentation (Abberior Instruments GmbH, Göttingen, Germany). For confocal and STED microscopy, a 100× Olympus UPlanSApo (NA 1.4) oil immersion objective was used. For excitation (λ= 594 nm and λ = 640 nm), a nominal laser power of 20% was applied, and for STED (λ = 775 nm, max. power = 1.2 W), a nominal laser power of 70 % was applied. The pixel size was set to 60 nm (.....
Document: Fluorescence imaging was performed using a two-colour-STED microscopy instrumentation (Abberior Instruments GmbH, Göttingen, Germany). For confocal and STED microscopy, a 100× Olympus UPlanSApo (NA 1.4) oil immersion objective was used. For excitation (λ= 594 nm and λ = 640 nm), a nominal laser power of 20% was applied, and for STED (λ = 775 nm, max. power = 1.2 W), a nominal laser power of 70 % was applied. The pixel size was set to 60 nm (confocal) and 15 nm (non-diffracted), respectively. Minor adjustments of contrast and brightness of the acquired images as well as the Richardson-Lucy deconvolution with a regularisation parameter of 0.001 (stopped after 30 iterations) were carried out with the imspector software (16.1.7098-win64-AIFpgaV3, Abberior Instruments GmbH, Göttingen, Germany).
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