Selected article for: "library sequencing and RNA library sequencing"
Author: Shifman, Ohad; Cohen-Gihon, Inbar; Beth-Din, Adi; Zvi, Anat; Laskar, Orly; Paran, Nir; Epstein, Eyal; Stein, Dana; Dorozko, Marina; Wolf, Dana; Yitzhaki, Shmuel; Shapira, Shmuel C.; Melamed, Sharon; Israeli, Ofir
Title: Identification and genetic characterization of a novel Orthobunyavirus species by a straightforward high-throughput sequencing-based approach
  • Document date: 2019_3_4
    • ID: 15cxc32n_17
    Snippet: The libraries were sequenced as single-end reads of 60 nucleotides. Sequencing reads of 60 bases enable short run times (<5 hours) and, in our hands, are sufficient for identification and characterization of RNA viruses, which have short and simple genomes, even when de novo assembly and reconstruction of the genome is required. The whole process, including RNA extraction, library preparation, sequencing and primary bioinformatic analysis, can be.....
    Document: The libraries were sequenced as single-end reads of 60 nucleotides. Sequencing reads of 60 bases enable short run times (<5 hours) and, in our hands, are sufficient for identification and characterization of RNA viruses, which have short and simple genomes, even when de novo assembly and reconstruction of the genome is required. The whole process, including RNA extraction, library preparation, sequencing and primary bioinformatic analysis, can be completed in less than 12 hours.

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