Author: van der Schaar, H. M.; Melia, C. E.; van Bruggen, J. A. C.; Strating, J. R. P. M.; van Geenen, M. E. D.; Koster, A. J.; Bárcena, M.; van Kuppeveld, F. J. M.
Title: Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein Document date: 2016_7_6
ID: 1aptufp6_18
Snippet: GFP(S1-10) (B) were infected with wild-type (wt) CVB3 or CVB3-3A(S11aa2) for 30 min at an MOI of 1. At the time points indicated, cells were subjected to titration analysis after freeze-thawing cycles to determine the amount of infectious virus particles. Alternatively, cells were lysed to determine the amount of viral RNA by quantitative PCR. The results are expressed as fold induction relative to the quantities determined directly after removin.....
Document: GFP(S1-10) (B) were infected with wild-type (wt) CVB3 or CVB3-3A(S11aa2) for 30 min at an MOI of 1. At the time points indicated, cells were subjected to titration analysis after freeze-thawing cycles to determine the amount of infectious virus particles. Alternatively, cells were lysed to determine the amount of viral RNA by quantitative PCR. The results are expressed as fold induction relative to the quantities determined directly after removing the inoculum.
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