Selected article for: "cell prrsv replication and prrsv replication"
Author: Takenouchi, Takato; Kitani, Hiroshi; Suzuki, Shunichi; Nakai, Michiko; Fuchimoto, Dai-ichiro; Tsukimoto, Mitsutoshi; Shinkai, Hiroki; Sato, Mitsuru; Uenishi, Hirohide
Title: Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase
  • Document date: 2017_8_21
    • ID: 0cqtxqjv_6
    Snippet: To promote virological and immunological studies in swine, several porcine macrophage cell lines have been developed (7) (8) (9) (10) . Among these, only three cell lines that were established by the transfection of primary porcine alveolar macrophages with the pSV3-neo plasmid, which carries genes for neomycin resistance and the SV40 large T antigen (SV40LT) (9) , are commercially available from the American Type Culture Collection (ATCC Number:.....
    Document: To promote virological and immunological studies in swine, several porcine macrophage cell lines have been developed (7) (8) (9) (10) . Among these, only three cell lines that were established by the transfection of primary porcine alveolar macrophages with the pSV3-neo plasmid, which carries genes for neomycin resistance and the SV40 large T antigen (SV40LT) (9) , are commercially available from the American Type Culture Collection (ATCC Number: CRL-2843, CRL-2844, and CRL-2845). However, it is reported that these cell lines did not support the replication of PRRSV (11) , suggesting that they do not possess all the features of primary alveolar macrophages. Thus, continuous efforts are being made to develop convenient porcine cell lines that retain the characteristics of primary macrophages.

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