Selected article for: "cell line and macrophage cell line"
Author: Takenouchi, Takato; Kitani, Hiroshi; Suzuki, Shunichi; Nakai, Michiko; Fuchimoto, Dai-ichiro; Tsukimoto, Mitsutoshi; Shinkai, Hiroki; Sato, Mitsuru; Uenishi, Hirohide
Title: Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase
  • Document date: 2017_8_21
    • ID: 0cqtxqjv_7
    Snippet: In our previous study, we isolated macrophages from a mixed primary culture of porcine kidney tissue (12) . Using these primary porcine kidney-derived macrophages (PKM), we attempted to produce an immortalized macrophage cell line in this study. As a result, we successfully generated a novel immortalized PKM (IPKM) cell line by transferring the SV40LT and porcine telomerase reverse transcriptase (pTERT) genes into PKM using lentiviral vectors. Ou.....
    Document: In our previous study, we isolated macrophages from a mixed primary culture of porcine kidney tissue (12) . Using these primary porcine kidney-derived macrophages (PKM), we attempted to produce an immortalized macrophage cell line in this study. As a result, we successfully generated a novel immortalized PKM (IPKM) cell line by transferring the SV40LT and porcine telomerase reverse transcriptase (pTERT) genes into PKM using lentiviral vectors. Our lentiviral transduction protocol might be a useful approach to the immortalization of primary porcine macrophages.

    Search related documents:
    Co phrase search for related documents
    • cell line and lentiviral transduction: 1, 2, 3, 4, 5
    • cell line and lentiviral transduction protocol: 1
    • cell line and lentiviral vector: 1, 2, 3, 4, 5, 6, 7, 8
    • cell line and macrophage cell line: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • cell line and porcine macrophage: 1, 2, 3, 4, 5, 6
    • cell line and previous study: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
    • cell line and primary culture: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    • cell line and primary porcine: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • cell line and primary porcine macrophage: 1
    • cell line and ptert gene: 1
    • cell line and reverse transcriptase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
    • IPKM cell line and lentiviral transduction: 1
    • IPKM cell line and macrophage cell line: 1, 2, 3
    • IPKM cell line and porcine macrophage: 1, 2, 3
    • IPKM cell line and previous study: 1
    • IPKM cell line and primary porcine: 1, 2, 3
    • IPKM cell line and ptert gene: 1
    • IPKM cell line and reverse transcriptase: 1, 2
    • lentiviral transduction and macrophage cell line: 1