Author: Takenouchi, Takato; Kitani, Hiroshi; Suzuki, Shunichi; Nakai, Michiko; Fuchimoto, Dai-ichiro; Tsukimoto, Mitsutoshi; Shinkai, Hiroki; Sato, Mitsuru; Uenishi, Hirohide
Title: Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase Document date: 2017_8_21
ID: 0cqtxqjv_7
Snippet: In our previous study, we isolated macrophages from a mixed primary culture of porcine kidney tissue (12) . Using these primary porcine kidney-derived macrophages (PKM), we attempted to produce an immortalized macrophage cell line in this study. As a result, we successfully generated a novel immortalized PKM (IPKM) cell line by transferring the SV40LT and porcine telomerase reverse transcriptase (pTERT) genes into PKM using lentiviral vectors. Ou.....
Document: In our previous study, we isolated macrophages from a mixed primary culture of porcine kidney tissue (12) . Using these primary porcine kidney-derived macrophages (PKM), we attempted to produce an immortalized macrophage cell line in this study. As a result, we successfully generated a novel immortalized PKM (IPKM) cell line by transferring the SV40LT and porcine telomerase reverse transcriptase (pTERT) genes into PKM using lentiviral vectors. Our lentiviral transduction protocol might be a useful approach to the immortalization of primary porcine macrophages.
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