Selected article for: "dna extraction and human dna"
Author: Jordana-Lluch, Elena; Giménez, Montserrat; Quesada, M. Dolores; Ausina, Vicente; Martró, Elisa
Title: Improving the Diagnosis of Bloodstream Infections: PCR Coupled with Mass Spectrometry
  • Document date: 2014_4_9
    • ID: 05zahl5n_12
    Snippet: The VYOO assay is a multiplexed PCR analysis that detects 34 pathogens, including six species of Candida and Aspergillus fumigatus, as well as several resistance genes (methicillin resistance gene mecA, vancomycin resistance genes vanA and vanB, and -lactamase genes blaSHV and blaCTX-M). The amplified products are visualized using a conventional gel electrophoresis, and the time-to-result is 8 hours. For this assay, microbial DNA from 5 mL of blo.....
    Document: The VYOO assay is a multiplexed PCR analysis that detects 34 pathogens, including six species of Candida and Aspergillus fumigatus, as well as several resistance genes (methicillin resistance gene mecA, vancomycin resistance genes vanA and vanB, and -lactamase genes blaSHV and blaCTX-M). The amplified products are visualized using a conventional gel electrophoresis, and the time-to-result is 8 hours. For this assay, microbial DNA from 5 mL of blood is enriched: total DNA is applied to an affinity chromatographic column that specifically binds the microbial DNA (LOOXTER) [27] . Additionally, human DNA is depleted during the extraction step. This assay has a sensitivity ranging from 38.0% to 60.0% [14, 25, 28] .

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