Selected article for: "luciferase activity and Promega assay kit"
Author: Wang, Xiaoli; Wang, Jiao; Zhang, Wenmei; Li, Boye; Zhu, Ying; Hu, Qin; Yang, Yishu; Zhang, Xiaoguang; Yan, Hong; Zeng, Yi
Title: Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate
  • Document date: 2018_5_16
    • ID: 1ghbutov_10
    Snippet: To assess the activity of PT-1 on HIV-1 virus entry, vesicular stomatitis virus glycoprotein (VSVG) pseudotyped viruses were produced by co-transfected HEK-293T cells with pCMV-VSVG and pSG3∆env plasmids. Virus titers were determined by the TCID 50 assay. The TZM-bl cells were then infected with either 1000 TCID 50 /ml Env-pseudotyped viruses or the equal amount of VSVG-pseudotyped viruses. After the addition of indicated doses of PT-1 and incu.....
    Document: To assess the activity of PT-1 on HIV-1 virus entry, vesicular stomatitis virus glycoprotein (VSVG) pseudotyped viruses were produced by co-transfected HEK-293T cells with pCMV-VSVG and pSG3∆env plasmids. Virus titers were determined by the TCID 50 assay. The TZM-bl cells were then infected with either 1000 TCID 50 /ml Env-pseudotyped viruses or the equal amount of VSVG-pseudotyped viruses. After the addition of indicated doses of PT-1 and incubation for 48 h, the luciferase activity was measured (Bright-Glo luciferase assay kit, Promega) to compare the difference between Env and VSVG-mediated virus entry inhibition.

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