Author: Lelli, Davide; Lavazza, Antonio; Prosperi, Alice; Sozzi, Enrica; Faccin, Francesca; Baioni, Laura; Trogu, Tiziana; Cavallari, Gian Luca; Mauri, Matteo; Gibellini, Anna Maria; Chiapponi, Chiara; Moreno, Ana
Title: Hypsugopoxvirus: A Novel Poxvirus Isolated from Hypsugo savii in Italy Document date: 2019_6_19
ID: 1axsebya_11
Snippet: Viral DNA was extracted from 200 µL of positive cell culture supernatants using a BioSprint 96 One-For-All Vet Kit (Qiagen S.p.A., Milan, Italy). Sequencing libraries were made with a Nextera Flex kit (Illumina Inc. San Diego, CA, USA) in accordance with the manufacturer's instructions. Libraries were sequenced on a MiSeq Instrument (Illumina Inc. San Diego, CA, USA) by using a MiSeq Reagent Kit v2 in a 250 cycle paired-end run. Data were assemb.....
Document: Viral DNA was extracted from 200 µL of positive cell culture supernatants using a BioSprint 96 One-For-All Vet Kit (Qiagen S.p.A., Milan, Italy). Sequencing libraries were made with a Nextera Flex kit (Illumina Inc. San Diego, CA, USA) in accordance with the manufacturer's instructions. Libraries were sequenced on a MiSeq Instrument (Illumina Inc. San Diego, CA, USA) by using a MiSeq Reagent Kit v2 in a 250 cycle paired-end run. Data were assembled de novo by the CLC Genomic workbench v.11 (Qiagen S.p.A., Milan, Italy).
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