Author: Takenouchi, Takato; Kitani, Hiroshi; Suzuki, Shunichi; Nakai, Michiko; Fuchimoto, Dai-ichiro; Tsukimoto, Mitsutoshi; Shinkai, Hiroki; Sato, Mitsuru; Uenishi, Hirohide
Title: Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase Document date: 2017_8_21
ID: 0cqtxqjv_23
Snippet: Immortalized porcine kidney-derived macrophages (5 × 10 5 ) were also cultured in 35-mm glass-bottomed dishes (Asahi Glass Co., Ltd.) containing growth medium. The next day, 20 µg/ml of pHrodo-labeled E. coli BioParticles (Thermo Fisher Scientific) were added, and the cells were subjected to time-lapse recording at 37°C for 4 h using an inverted fluorescence microscope (Olympus IX-81, Tokyo, Japan). The intensity of the fluorescence emitted by.....
Document: Immortalized porcine kidney-derived macrophages (5 × 10 5 ) were also cultured in 35-mm glass-bottomed dishes (Asahi Glass Co., Ltd.) containing growth medium. The next day, 20 µg/ml of pHrodo-labeled E. coli BioParticles (Thermo Fisher Scientific) were added, and the cells were subjected to time-lapse recording at 37°C for 4 h using an inverted fluorescence microscope (Olympus IX-81, Tokyo, Japan). The intensity of the fluorescence emitted by the pHrodo was quantified based on the captured photographs using the MetaMorph software version 7.6 (Molecular Devices, Downingtown, PA, USA).
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