Selected article for: "culture supernatant and growth medium"
Author: Takenouchi, Takato; Kitani, Hiroshi; Suzuki, Shunichi; Nakai, Michiko; Fuchimoto, Dai-ichiro; Tsukimoto, Mitsutoshi; Shinkai, Hiroki; Sato, Mitsuru; Uenishi, Hirohide
Title: Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase
  • Document date: 2017_8_21
    • ID: 0cqtxqjv_25
    Snippet: The macrophages were seeded in 60-mm plastic dishes at a density of 1 × 10 6 cells/dish. The next day, the medium was replaced by growth medium containing lipopolysaccharide (LPS) (from E. coli serotype O127:B8, Sigma L3129) at doses of 0.1-1.0 µg/ml. After the cells had been incubated for 24 h at 37°C, the culture supernatant was collected, filtered with a membrane filter (pore size: 0.45 µm, Millipore Millex), and stored at −80°C until u.....
    Document: The macrophages were seeded in 60-mm plastic dishes at a density of 1 × 10 6 cells/dish. The next day, the medium was replaced by growth medium containing lipopolysaccharide (LPS) (from E. coli serotype O127:B8, Sigma L3129) at doses of 0.1-1.0 µg/ml. After the cells had been incubated for 24 h at 37°C, the culture supernatant was collected, filtered with a membrane filter (pore size: 0.45 µm, Millipore Millex), and stored at −80°C until use. Aliquots of the samples were assayed using porcine cytokine enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems, Minneapolis, MN, USA), according to the manufacturer's instructions. The experiments were independently performed three times, and the cytokine concentrations of the culture supernatant are expressed as mean ± SEM values.

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    • µg ml and sample aliquot: 1
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    • ELISA kit and USA MN Minneapolis Systems ELISA kit: 1
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    • immunosorbent assay and USA MN Minneapolis Systems ELISA kit: 1
    • mean SEM value and SEM value: 1, 2
    • membrane filter and pore size: 1, 2, 3
    • Millipore Millex µm pore size and pore size: 1