Selected article for: "genomic sequence and wild type"
Author: Takenouchi, Takato; Kitani, Hiroshi; Suzuki, Shunichi; Nakai, Michiko; Fuchimoto, Dai-ichiro; Tsukimoto, Mitsutoshi; Shinkai, Hiroki; Sato, Mitsuru; Uenishi, Hirohide
Title: Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase
  • Document date: 2017_8_21
    • ID: 0cqtxqjv_29
    Snippet: To discriminate between wild type and LDLR-KO pig-derived macrophages, PCR analyses were performed using KOD-FX (Toyobo, Tokyo, Japan), according to the manufacturer's instructions. The cells (5 × 10 4 ) were directly introduced into the PCR reaction. The following oligonucleotide primers were used: porcine LDLR: sense, 5′-GCAAGATAGGGGACTTTAGC-3′, and antisense, 5′-GCAAGATAGGGGACTTTAGC-3′; FP2: antisense, 5′-ACCAAATTAAGGGCCAGCTC-3′. .....
    Document: To discriminate between wild type and LDLR-KO pig-derived macrophages, PCR analyses were performed using KOD-FX (Toyobo, Tokyo, Japan), according to the manufacturer's instructions. The cells (5 × 10 4 ) were directly introduced into the PCR reaction. The following oligonucleotide primers were used: porcine LDLR: sense, 5′-GCAAGATAGGGGACTTTAGC-3′, and antisense, 5′-GCAAGATAGGGGACTTTAGC-3′; FP2: antisense, 5′-ACCAAATTAAGGGCCAGCTC-3′. The FP2 primer was designed based on a sequence within the targeted genomic region of LDLR-KO pigs (15) . In addition, DNA fragments containing the targeted genomic region were amplified by PCR and cloned into the pBluescript cloning vector, and the vector plasmid was used

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