Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase

Takenouchi, Takato; Kitani, Hiroshi; Suzuki, Shunichi; Nakai, Michiko; Fuchimoto, Dai-ichiro; Tsukimoto, Mitsutoshi; Shinkai, Hiroki; Sato, Mitsuru; Uenishi, Hirohide

The cultured IPKM exhibited a typical macrophage-like morphology with ruffled membranes and cell processes ( Figure 1B); i.e., they resembled primary PKM (Figure 1A) . Immunostaining demonstrated that the IPKM were positive for macrophage markers (Iba1, KT022, and CD172a), but negative for epithelial (CK18 and CK19) and mesenchymal (SMA) cell markers (Figure 2A) , as was observed in the primary PKM (12) . Regarding other cell surface markers of macrophages, although IPKM seem to express CD16 and major histocompatibility complex class II ( Figure S1 in Supplementary Material), further experiments will be required to reveal the marker expression profiles of these cells. The IPKM proliferated at a doubling time of around 4 days and were stably passaged for >40 population doublings (Figure 2B ).