Author: Takenouchi, Takato; Kitani, Hiroshi; Suzuki, Shunichi; Nakai, Michiko; Fuchimoto, Dai-ichiro; Tsukimoto, Mitsutoshi; Shinkai, Hiroki; Sato, Mitsuru; Uenishi, Hirohide
Title: Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase Document date: 2017_8_21
ID: 0cqtxqjv_42
Snippet: This study describes a novel immortalized porcine macrophage cell line, IPKM, which was produced through the transduction of primary PKM with lentiviral vectors encoding SV40LT and pTERT. Using the same lentiviral transduction system, primary macrophages that were isolated from the peripheral blood of an LDLR-KO pig were successfully immortalized, suggesting that this protocol could be used to immortalize primary porcine macrophages. Since the IP.....
Document: This study describes a novel immortalized porcine macrophage cell line, IPKM, which was produced through the transduction of primary PKM with lentiviral vectors encoding SV40LT and pTERT. Using the same lentiviral transduction system, primary macrophages that were isolated from the peripheral blood of an LDLR-KO pig were successfully immortalized, suggesting that this protocol could be used to immortalize primary porcine macrophages. Since the IPKM retained the characteristics of primary macrophages, this cell line could be used to develop in vitro cellular models for studying genes associated with innate immunity in swine and porcine pathogen infection processes.
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