Author: Chenoll, Empar; Casinos, Beatriz; Bataller, Esther; Buesa, Javier; Ramón, Daniel; Genovés, Salvador; Fábrega, Joan; Rivero Urgell, Montserrat; Moreno Muñoz, José A.
Title: Identification of a Peptide Produced by Bifidobacterium longum CECT 7210 with Antirotaviral Activity Document date: 2016_5_4
ID: 0sxl6f1r_37
Snippet: The strategy used to identify the active substance against rotavirus started with its purification from the CECT 7210 active supernatant. To do this, a total of 28 fractions of reverse phase were selected for rotavirus inhibition assays (strategy B). Of these, only six fractions rendered a significant reduction in infected foci and were selected for further investigation. The selected fractions are summarized in Table 3 . All the fractions that p.....
Document: The strategy used to identify the active substance against rotavirus started with its purification from the CECT 7210 active supernatant. To do this, a total of 28 fractions of reverse phase were selected for rotavirus inhibition assays (strategy B). Of these, only six fractions rendered a significant reduction in infected foci and were selected for further investigation. The selected fractions are summarized in Table 3 . All the fractions that proved positive for inhibition were analyzed by MALDI-TOF peptide mass fingerprinting to determine the molecular weights of the peptide/s present in each fraction (results of each fraction are not shown). Analyzing all the spectra, the only common signal in all Results are the average of at least two independent experiments. SD, standard deviation; a P-value < 0.05; b P-value < 0.001.
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