Selected article for: "FACS flow cytometry and flow cytometry"
Author: He, Chaobin; Wang, Jun; Sun, Shuxin; Zhang, Yu; Li, Shengping
Title: Immunomodulatory Effect after Irreversible Electroporation in Patients with Locally Advanced Pancreatic Cancer
  • Document date: 2019_5_12
    • ID: 1pbzbydu_9
    Snippet: . . Flow Cytometry Analysis. Frozen PBMCs were thawed in a 37 ∘ C water bath and then cultured overnight at 37 ∘ C in RPMI-1640 (Gibco BRL) supplemented with 5% human AB type serum and labeled with FITC-, APC-, and/or PEconjugated murine anti-human monoclonal antibodies. The CD3, CD3CD4, CD3CD8, CD3CD16CD56, and CD4CD25 phenotype of lymphocytes were sequentially analyzed by flow cytometry (FACS caliber, 4 color system, BD Bioscience, CA, US)......
    Document: . . Flow Cytometry Analysis. Frozen PBMCs were thawed in a 37 ∘ C water bath and then cultured overnight at 37 ∘ C in RPMI-1640 (Gibco BRL) supplemented with 5% human AB type serum and labeled with FITC-, APC-, and/or PEconjugated murine anti-human monoclonal antibodies. The CD3, CD3CD4, CD3CD8, CD3CD16CD56, and CD4CD25 phenotype of lymphocytes were sequentially analyzed by flow cytometry (FACS caliber, 4 color system, BD Bioscience, CA, US).

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