Selected article for: "broad range and human cell"
Author: Jordana-Lluch, Elena; Giménez, Montserrat; Quesada, M. Dolores; Ausina, Vicente; Martró, Elisa
Title: Improving the Diagnosis of Bloodstream Infections: PCR Coupled with Mass Spectrometry
  • Document date: 2014_4_9
    • ID: 05zahl5n_9
    Snippet: The SepsiTest assay is based on broad-range PCR amplification followed by sequencing. In the SepsiTest two 1 mL aliquots of blood are processed in duplicate and human DNA is selectively degraded prior to the bacterial cell lysis step [16] . Several studies using this approach for the diagnosis of sepsis have been published. The largest study ( = 342) [16] reported a sensitivity and specificity of 87.0% and 85.8%. Two smaller studies reported lowe.....
    Document: The SepsiTest assay is based on broad-range PCR amplification followed by sequencing. In the SepsiTest two 1 mL aliquots of blood are processed in duplicate and human DNA is selectively degraded prior to the bacterial cell lysis step [16] . Several studies using this approach for the diagnosis of sepsis have been published. The largest study ( = 342) [16] reported a sensitivity and specificity of 87.0% and 85.8%. Two smaller studies reported lower values of sensitivity of 46.0% ( = 50)

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