Author: Wang, Xiaoli; Wang, Jiao; Zhang, Wenmei; Li, Boye; Zhu, Ying; Hu, Qin; Yang, Yishu; Zhang, Xiaoguang; Yan, Hong; Zeng, Yi
Title: Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate Document date: 2018_5_16
ID: 1ghbutov_40
Snippet: We further examined the ability of PT-1 to inhibit the viral protease, reverse transcriptase and integrase. Pepstatin A, nevirapine (NVP), and RAL were used as positive controls, respectively. PT-1 did not suppress protease activity ( Figure 5A ) but blocked reverse transcriptase activity ( Figure 5B ) and HIV-1 integrase 3′ processing ( Figure 5C ) only at the highest concentration (17.6 µM). The maximum inhibition rates of reverse transcript.....
Document: We further examined the ability of PT-1 to inhibit the viral protease, reverse transcriptase and integrase. Pepstatin A, nevirapine (NVP), and RAL were used as positive controls, respectively. PT-1 did not suppress protease activity ( Figure 5A ) but blocked reverse transcriptase activity ( Figure 5B ) and HIV-1 integrase 3′ processing ( Figure 5C ) only at the highest concentration (17.6 µM). The maximum inhibition rates of reverse transcriptase and integrase 3′ processing were approximately 70% and 60%, respectively. These data implied that in vitro reduction of reverse transcriptase and integrase activity was unlikely to be major mechanism of PT-1 induced HIV-1 inhibition at nanomolar concentrations.
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