Author: Rhein, Bethany A.; Powers, Linda S.; Rogers, Kai; Anantpadma, Manu; Singh, Brajesh K.; Sakurai, Yasuteru; Bair, Thomas; Miller-Hunt, Catherine; Sinn, Patrick; Davey, Robert A.; Monick, Martha M.; Maury, Wendy
Title: Interferon-? Inhibits Ebola Virus Infection Document date: 2015_11_12
ID: 10bu7iwg_48
Snippet: The University of Iowa Institutional Review Board (IRB) approved all procedures for blood draws after obtaining informed consent from all individuals and all studies involving humans were performed under the approved University of Iowa IRB protocol #200607708 to Dr. Monick. Peripheral blood mononuclear cells (PBMCs) were isolated from human blood using Ficoll-Hypaque (Sigma-Aldrich), and monocyte-derived macrophages (MDMs) were isolated by adhere.....
Document: The University of Iowa Institutional Review Board (IRB) approved all procedures for blood draws after obtaining informed consent from all individuals and all studies involving humans were performed under the approved University of Iowa IRB protocol #200607708 to Dr. Monick. Peripheral blood mononuclear cells (PBMCs) were isolated from human blood using Ficoll-Hypaque (Sigma-Aldrich), and monocyte-derived macrophages (MDMs) were isolated by adherence as previously described [88] . Isolated MDMs were plated in Dulbecco's modified Eagle medium (DMEM) (Gibco) media containing 10% fetal bovine serum (FBS) (Atlanta Biologicals), 10% Type AB human serum (Sigma), 1% penicillin/streptomycin (P/S) (Life Technologies) and 50 ng/mL of human M-CSF (R&D Systems). MDMs were differentiated for 6 days. After 6 days, media on the MDM cultures was replaced with media containing human M-CSF alone with or without the addition of 20 ng/mL of IFNγ and/or TNFα (R&D Systems), unless otherwise indicated in the figure legend.
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