Author: Rhein, Bethany A.; Powers, Linda S.; Rogers, Kai; Anantpadma, Manu; Singh, Brajesh K.; Sakurai, Yasuteru; Bair, Thomas; Miller-Hunt, Catherine; Sinn, Patrick; Davey, Robert A.; Monick, Martha M.; Maury, Wendy
Title: Interferon-? Inhibits Ebola Virus Infection Document date: 2015_11_12
ID: 10bu7iwg_74
Snippet: EBOV GP/rVSV infections. Organs were harvested 2 days following infection from at least 4 mice from each treatment group. Mice were anesthetized with isofluorene to perform retro-orbital bleeds for serum. Mice were euthanized and perfused with 10 mL of PBS through the heart prior to harvesting organs. The total peritoneal cell population was isolated and lysed for qRT-PCR to detect viral RNA as described above. Organs were homogenized in PBS and .....
Document: EBOV GP/rVSV infections. Organs were harvested 2 days following infection from at least 4 mice from each treatment group. Mice were anesthetized with isofluorene to perform retro-orbital bleeds for serum. Mice were euthanized and perfused with 10 mL of PBS through the heart prior to harvesting organs. The total peritoneal cell population was isolated and lysed for qRT-PCR to detect viral RNA as described above. Organs were homogenized in PBS and titers were determined by end-point dilution as described above on mycoplasma-free Vero cells.
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