Author: van der Schaar, H. M.; Melia, C. E.; van Bruggen, J. A. C.; Strating, J. R. P. M.; van Geenen, M. E. D.; Koster, A. J.; Bárcena, M.; van Kuppeveld, F. J. M.
Title: Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein Document date: 2016_7_6
ID: 1aptufp6_21
Snippet: To ensure that GFP fluorescence is emitted exclusively from 3A(S11aa2)-containing sites, cells from the same infection were stained with an anti-3A antibody. Figure 3B shows that GFP fluorescence was detected in a pattern that greatly overlapped with 3A visualized by immunofluorescence. However, the immunofluorescence with the 3A antibody generated a slightly more extensive 3A pattern, most likely as a consequence of not all 3A(S11aa2) proteins b.....
Document: To ensure that GFP fluorescence is emitted exclusively from 3A(S11aa2)-containing sites, cells from the same infection were stained with an anti-3A antibody. Figure 3B shows that GFP fluorescence was detected in a pattern that greatly overlapped with 3A visualized by immunofluorescence. However, the immunofluorescence with the 3A antibody generated a slightly more extensive 3A pattern, most likely as a consequence of not all 3A(S11aa2) proteins being bound by GFP(S1-10). This could be due to partial inaccessibility of 3A(S11aa2) proteins to their GFP(S1-10) counterparts, as 3A proteins localize to densely packed ROs.
Search related documents:
Co phrase search for related documents- bind protein and GFP fluorescence: 1, 2
- bind protein and infection cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- GFP fluorescence and infection cell: 1, 2, 3, 4
Co phrase search for related documents, hyperlinks ordered by date