Author: van der Schaar, H. M.; Melia, C. E.; van Bruggen, J. A. C.; Strating, J. R. P. M.; van Geenen, M. E. D.; Koster, A. J.; Bárcena, M.; van Kuppeveld, F. J. M.
Title: Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein Document date: 2016_7_6
ID: 1aptufp6_26
Snippet: show that 3A accumulation coincides with the local disruption of Golgi morphology, leading to global fragmentation and eventual disassembly of the Golgi apparatus. After complete Golgi fragmentation, the 3A-GFP signal expands throughout the cell and eventually occupies the entire cytoplasm before the cell goes into demise, demonstrating that 3A-GFP signals can be imaged to the point of cell lysis (Movies S1 and S2)......
Document: show that 3A accumulation coincides with the local disruption of Golgi morphology, leading to global fragmentation and eventual disassembly of the Golgi apparatus. After complete Golgi fragmentation, the 3A-GFP signal expands throughout the cell and eventually occupies the entire cytoplasm before the cell goes into demise, demonstrating that 3A-GFP signals can be imaged to the point of cell lysis (Movies S1 and S2).
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