Author: Wang, Xiaoli; Wang, Jiao; Zhang, Wenmei; Li, Boye; Zhu, Ying; Hu, Qin; Yang, Yishu; Zhang, Xiaoguang; Yan, Hong; Zeng, Yi
Title: Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate Document date: 2018_5_16
ID: 1ghbutov_20
Snippet: The cell apoptosis of PBMCs were detected using Annexin V, FITC Apoptosis Detection Kit (Dojindo, Tokyo, Japan). PBMCs were isolated and activated with ConA (5 µg/mL, Sigma) for 2 days as described. Pre-stimulated PBMC (1 × 10 5 /well) were then incubated with PT-1 (1.76, 8.80, and 44.00 µM) in fresh RPMI1640 (containing 25 ng/mL IL-2) for another 3 days. On day 5, cells were collected and stained with Annexin-V-FITC and propidium iodide (PI) .....
Document: The cell apoptosis of PBMCs were detected using Annexin V, FITC Apoptosis Detection Kit (Dojindo, Tokyo, Japan). PBMCs were isolated and activated with ConA (5 µg/mL, Sigma) for 2 days as described. Pre-stimulated PBMC (1 × 10 5 /well) were then incubated with PT-1 (1.76, 8.80, and 44.00 µM) in fresh RPMI1640 (containing 25 ng/mL IL-2) for another 3 days. On day 5, cells were collected and stained with Annexin-V-FITC and propidium iodide (PI) following manufacturer's instruction. All data were acquired with a BD FACS Calibur flow cytometer (BD Biosciences, San Jose, CA, USA).
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