Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate

Wang, Xiaoli; Wang, Jiao; Zhang, Wenmei; Li, Boye; Zhu, Ying; Hu, Qin; Yang, Yishu; Zhang, Xiaoguang; Yan, Hong; Zeng, Yi

TZM-bl cells were obtained from the National Institutes of Health AIDS Reagent Repository and cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco, Shanghai, China) supplemented with 10% (v/v) fetal bovine serum (FBS; Hyclone, Shanghai, China), 200 µg/mL L-glutamine and 100 U/mL penicillin-streptomycin. HEK-293T cells and the human T-cell line MT4 was purchased from American Type Culture Collection (ATCC) and maintained in RPMI 1640 (Gibco) supplemented with 10% (v/v) fetal bovine serum, 200 µg/mL L-glutamine and 100 U/mL penicillin-streptomycin. Peripheral blood mononuclear cells (PBMC) were isolated from three healthy donors and cultured in complete RPMI medium (10% fetal bovine serum, 200 µg/mL L-glutamine, 100 U/mL penicillin-streptomycin, and 25 ng/mL interleukin-2 (IL-2)). The HIV Env clones Q842env.d16 (NIH#11886), Q259env.w6 (NIH#11887), TRO.11 (NIH#11023), REJO4541.67 (NIH#11035), SC422661.8 (NIH#11058), Du422.1 (NIH#11308), QA790.204I.ENV.A4 (NIH#11901), QA790.204I.ENV.C1 (NIH#11902), and backbone vector pSG3∆env (NIH#11051) were obtained from the National Institutes of Health AIDS Reagent Repository. BJOX00200 env (NIH#12655), CH119 env (NIH#12659), CE1176 env (NIH#12657), and CNE8 env (NIH#12653) were kindly provided by Dr. Lei Yu (Shenzhen CDC, China). HIV-1 NL4-3, HXB2, and BH10 strains were kindly gifted from Institute of Biophysics, Chinese Academy of Sciences. pCMV-VSV-G Envelope Vector was kindly gifted by Dr. Rui Pedro Galao (King's College London, London, UK). PT-1(K 6 HPTi 2 W 10 O 40 ) was synthesized by Dr. Yan Hong as previously described [28, 29] . The structure information of PT-1 was deposited at the Cambridge Crystallographic Data Centre (CCDC: 1440344). The aqueous stock solution of PT-1 (3.5 mM) was stored at −20 • C.