Author: Kumar, Satyendra; Arankalle, Vidya A.
Title: Intracranial Administration of P Gene siRNA Protects Mice from Lethal Chandipura Virus Encephalitis Document date: 2010_1_7
ID: 1mzq227n_10
Snippet: All CHPV sequences present in the GenBank [23] and the sequences of the new isolates generated in the lab (NIV, unpublished data) were used to design the siRNA. All siRNAs were designed using HP OnGuard siRNA design. (Table 1 and Fig 1) . Four and 8 siRNAs were synthesized for P gene and M gene respectively targeting cDNA (+) strand. Negative control siRNA (ncsiRNA) with no significant homology to any known mammalian gene was used as a non-silenc.....
Document: All CHPV sequences present in the GenBank [23] and the sequences of the new isolates generated in the lab (NIV, unpublished data) were used to design the siRNA. All siRNAs were designed using HP OnGuard siRNA design. (Table 1 and Fig 1) . Four and 8 siRNAs were synthesized for P gene and M gene respectively targeting cDNA (+) strand. Negative control siRNA (ncsiRNA) with no significant homology to any known mammalian gene was used as a non-silencing control in all RNAi experiments and purchased from qiagen (Catalog number 1027280) and negative control plasmid (NCP) was used in shRNA experiments (Ambion, USA).
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