Author: Kumar, Satyendra; Arankalle, Vidya A.
Title: Intracranial Administration of P Gene siRNA Protects Mice from Lethal Chandipura Virus Encephalitis Document date: 2010_1_7
ID: 1mzq227n_20
Snippet: For plaque assay Vero cells were grown to near confluence in 24-well tissue culture plate. The cells were adsorbed with 10-fold serially diluted supernatant containing CHPV at 37uC for 60 min. Following adsorption, the cells were washed with PBS and fed with 1 ml of 2X MEM (Sigma, USA) along with 1.25% (w/v) carboxymethyl cellulose (Sigma, USA). The cells were incubated for 2 days and observed for plaques under an inverted microscope and stained .....
Document: For plaque assay Vero cells were grown to near confluence in 24-well tissue culture plate. The cells were adsorbed with 10-fold serially diluted supernatant containing CHPV at 37uC for 60 min. Following adsorption, the cells were washed with PBS and fed with 1 ml of 2X MEM (Sigma, USA) along with 1.25% (w/v) carboxymethyl cellulose (Sigma, USA). The cells were incubated for 2 days and observed for plaques under an inverted microscope and stained with crystal violet (0.1%) after fixing in 10% (v/v) formal saline. The plaques were counted and the titer of the virus was calculated as PFU/ml [25] .
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