Selected article for: "genomic dna and PCR amplification"
Author: Jordana-Lluch, Elena; Giménez, Montserrat; Quesada, M. Dolores; Ausina, Vicente; Martró, Elisa
Title: Improving the Diagnosis of Bloodstream Infections: PCR Coupled with Mass Spectrometry
  • Document date: 2014_4_9
    • ID: 05zahl5n_4
    Snippet: Use of whole blood in assays designed to detect pathogen nucleic acid is challenging. An excess of human DNA may hamper the detection of pathogen genomic material or may inhibit the PCR reaction [18, 19] ; hemoglobin traces may also inhibit PCR-based amplification. Therefore, molecular methods are forced to use a relatively small volume of blood (1 to 5 mL); whereas conventional culture methods use 20-30 mL. This limited volume reduces sensitivit.....
    Document: Use of whole blood in assays designed to detect pathogen nucleic acid is challenging. An excess of human DNA may hamper the detection of pathogen genomic material or may inhibit the PCR reaction [18, 19] ; hemoglobin traces may also inhibit PCR-based amplification. Therefore, molecular methods are forced to use a relatively small volume of blood (1 to 5 mL); whereas conventional culture methods use 20-30 mL. This limited volume reduces sensitivity of the molecular methods. Additionally, the bacterial load in adults with bloodstream infection can be as low as 1-10 CFU/mL [20] , which may preclude detection of pathogen DNA.

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