Author: Rhein, Bethany A.; Powers, Linda S.; Rogers, Kai; Anantpadma, Manu; Singh, Brajesh K.; Sakurai, Yasuteru; Bair, Thomas; Miller-Hunt, Catherine; Sinn, Patrick; Davey, Robert A.; Monick, Martha M.; Maury, Wendy
Title: Interferon-? Inhibits Ebola Virus Infection Document date: 2015_11_12
ID: 10bu7iwg_75
Snippet: MA-EBOV infections. Four days following infection, three animals per group were sacrificed, sera was isolated from blood samples and stored at -80°C until use. Virus loads were quantified by culturing 10-fold serial dilutions of serum in PBS on mycoplasma free Vero-E6 cells seeded in 6 well plates. After a one hour incubation, the supernatant was replaced with DMEM containing methylcellulose with 2% FBS and cells were incubated at 37°C for 10 d.....
Document: MA-EBOV infections. Four days following infection, three animals per group were sacrificed, sera was isolated from blood samples and stored at -80°C until use. Virus loads were quantified by culturing 10-fold serial dilutions of serum in PBS on mycoplasma free Vero-E6 cells seeded in 6 well plates. After a one hour incubation, the supernatant was replaced with DMEM containing methylcellulose with 2% FBS and cells were incubated at 37°C for 10 days. Cells were fixed in 10% formalin overnight and stained with crystal violet. Plaques were manually counted.
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