Author: Wang, Xiaoli; Wang, Jiao; Zhang, Wenmei; Li, Boye; Zhu, Ying; Hu, Qin; Yang, Yishu; Zhang, Xiaoguang; Yan, Hong; Zeng, Yi
Title: Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate Document date: 2018_5_16
ID: 1ghbutov_19
Snippet: The interaction of PT-1 and CD4 receptor was assessed by flow cytometry (FACS) analysis. Briefly, MT-4 cells were harvested and incubated with or without PT-1 (0.7, 3.5, and 17.6 µM) or gp120 (0.84 µM, positive control) for 30 min. Cell were then washed with FACS buffer and stained with PE anti-human CD4 antibody (BD Pharmingen, Shanghai, China) for 30 min on ice. After washing, the cells were resuspended in the FACS buffer for FACS analysis (B.....
Document: The interaction of PT-1 and CD4 receptor was assessed by flow cytometry (FACS) analysis. Briefly, MT-4 cells were harvested and incubated with or without PT-1 (0.7, 3.5, and 17.6 µM) or gp120 (0.84 µM, positive control) for 30 min. Cell were then washed with FACS buffer and stained with PE anti-human CD4 antibody (BD Pharmingen, Shanghai, China) for 30 min on ice. After washing, the cells were resuspended in the FACS buffer for FACS analysis (BD FACS Calibur flow cytometer, BD Biosciences, Franklin Lakes, NJ, USA).
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