Author: Wang, Xiaoli; Wang, Jiao; Zhang, Wenmei; Li, Boye; Zhu, Ying; Hu, Qin; Yang, Yishu; Zhang, Xiaoguang; Yan, Hong; Zeng, Yi
Title: Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate Document date: 2018_5_16
ID: 1ghbutov_5
Snippet: The cell counting kit-8 assay (CCK-8 assay, Dojindo, Shanghai, China) was performed to determine the cell viability of TZM-bl, HEK-293T and MT4 cells after PT-1 treatment. The cells seeded in a 96-well plate were treated with a serial dilution of PT-1 ranging from 3.5 to 70 µM for 48 h. At the end of experiment, cells were incubated with CCK-8 solution for 1 h and measured at 450 nm using the Enspire ® Multi-mode Plate Reader (PerkinElmer, Walt.....
Document: The cell counting kit-8 assay (CCK-8 assay, Dojindo, Shanghai, China) was performed to determine the cell viability of TZM-bl, HEK-293T and MT4 cells after PT-1 treatment. The cells seeded in a 96-well plate were treated with a serial dilution of PT-1 ranging from 3.5 to 70 µM for 48 h. At the end of experiment, cells were incubated with CCK-8 solution for 1 h and measured at 450 nm using the Enspire ® Multi-mode Plate Reader (PerkinElmer, Waltham, MI, USA). The cytotoxicity (CC 50 values) was calculated as 50% cytotoxic concentration using GraphPad Prism 6 software (version 6.01, GraphPad Software Inc., La Jolla, CA, USA). The selectivity index was calculated by CC 50 /IC 50 (The half maximal inhibitory concentration).
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