Author: Van der Gucht, Winke; Stobbelaar, Kim; Govaerts, Matthias; Mangodt, Thomas; Barbezange, Cyril; Leemans, Annelies; De Winter, Benedicte; Van Gucht, Steven; Caljon, Guy; Maes, Louis; De Dooy, Jozef; Jorens, Philippe; Smet, Annemieke; Cos, Paul; Verhulst, Stijn; Delputte, Peter L.
Title: Isolation and Characterization of Clinical RSV Isolates in Belgium during the Winters of 2016–2018 Document date: 2019_11_6
ID: 0imlae98_15
Snippet: HEp-2, A549 and BEAS-2B cells were seeded at a concentration of 200,000 cells/well in 24-well plates 24 h prior to infection. Briefly, before infection, cells were washed with DMEM-0 and afterwards infected with clinical isolates and RSV A2 and RSV B1 at a MOI of 0.01. The supernatant was collected after 24 h, 48 h and 72 h, aliquoted, snap frozen and stored at -80 • C. The supernatant was quantified using a conventional plaque assay on HEp-2 c.....
Document: HEp-2, A549 and BEAS-2B cells were seeded at a concentration of 200,000 cells/well in 24-well plates 24 h prior to infection. Briefly, before infection, cells were washed with DMEM-0 and afterwards infected with clinical isolates and RSV A2 and RSV B1 at a MOI of 0.01. The supernatant was collected after 24 h, 48 h and 72 h, aliquoted, snap frozen and stored at -80 • C. The supernatant was quantified using a conventional plaque assay on HEp-2 cells as described above.
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