Author: Wang, Xiaoli; Wang, Jiao; Zhang, Wenmei; Li, Boye; Zhu, Ying; Hu, Qin; Yang, Yishu; Zhang, Xiaoguang; Yan, Hong; Zeng, Yi
Title: Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate Document date: 2018_5_16
ID: 1ghbutov_15
Snippet: Viruses 2018, 10, 265 4 of 15 (5 × 10 4 TCID 50 ) and increasing concentrations of PT-1 (0.007, 0.027, 0.11, 0.44, 1.76, 3.52, and 7.04 µM) or AZT (0.004, 0.037, 0.37, and 3.74 µM) for 3 h at 37 • C. Infected cells were washed twice and added to 96-well plates containing the fresh PT-1 or AZT solution. On days 3, 5, 7 post-infection, half of the medium was removed and replaced with fresh medium containing PT-1 or AZT. Supernatants were colle.....
Document: Viruses 2018, 10, 265 4 of 15 (5 × 10 4 TCID 50 ) and increasing concentrations of PT-1 (0.007, 0.027, 0.11, 0.44, 1.76, 3.52, and 7.04 µM) or AZT (0.004, 0.037, 0.37, and 3.74 µM) for 3 h at 37 • C. Infected cells were washed twice and added to 96-well plates containing the fresh PT-1 or AZT solution. On days 3, 5, 7 post-infection, half of the medium was removed and replaced with fresh medium containing PT-1 or AZT. Supernatants were collected at days 3, 5, 7, and 9 after infection and tested for p24 antigen (Vironostika HIV-1 antigen ELISA kit). AZT was used as positive control.
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