Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate

Wang, Xiaoli; Wang, Jiao; Zhang, Wenmei; Li, Boye; Zhu, Ying; Hu, Qin; Yang, Yishu; Zhang, Xiaoguang; Yan, Hong; Zeng, Yi

A time-of-addition study was conducted using a single-round replication assay. The TZM-bl cells were infected with HIV-Env-pseudotyped viruses (REJO4541.67) for 2 h, then the cells were washed extensively to remove supernatant, and the fresh product was added to continue the experiment. PT-1 (1.7 µM) was added to the culture medium at 0 h, 0.25 h, 0.5 h, 1 h, 2 h, 6 h, 9 h and 24 h after infection, and maraviroc (MVC, 1 µM, CCR5 receptor antagonist), azidothymidine (AZT, 1 µM, Reverse-transcriptase inhibitor), raltegravir (RAL, 1 µM, integrase inhibitor), and T20 (1 µM, fusion inhibitor) severed as controls. At the end of the experiments, the inhibition of viral replication was measured by luciferase activity.