Interferon-? Inhibits Ebola Virus Infection

Rhein, Bethany A.; Powers, Linda S.; Rogers, Kai; Anantpadma, Manu; Singh, Brajesh K.; Sakurai, Yasuteru; Bair, Thomas; Miller-Hunt, Catherine; Sinn, Patrick; Davey, Robert A.; Monick, Martha M.; Maury, Wendy

Since experiments under BSL4 containment are hazardous, difficult and expensive, the BSL2 model virus, recombinant infectious vesicular stomatitis virus (rVSV) expressing EBOV GP (EBOV GP/rVSV), is frequently used to study a number of aspects of filovirus biology [21] [22] [23] [24] [25] . Infection of peritoneal macrophages with EBOV GP/rVSV resulted in remarkably similar effects of GM-CSF, M-CSF and the combination IFNγ/TNFα treatments to that we observed with EBOV (compare Fig 1A-1B and S2 Fig) . Thus, throughout these investigations, many of our studies were initially performed with EBOV GP/rVSV and subsequently confirmed and extended with EBOV in a BSL4 setting.