Author: Rhein, Bethany A.; Powers, Linda S.; Rogers, Kai; Anantpadma, Manu; Singh, Brajesh K.; Sakurai, Yasuteru; Bair, Thomas; Miller-Hunt, Catherine; Sinn, Patrick; Davey, Robert A.; Monick, Martha M.; Maury, Wendy
Title: Interferon-? Inhibits Ebola Virus Infection Document date: 2015_11_12
ID: 10bu7iwg_63
Snippet: ISG-RFP lentiviral constructs were a kind gift from Dr. Charles Rice (Rockerfeller University) and have been previously described [40] . HEK 293T cells stably expressing TIM-1 (H3 cells) [23] , which are routinely tested for the absence of mycoplasma, were transfected with the ISG-RFP constructs at the indicated concentrations. Forty-eight hours following transfection, cells were infected with EBOV-GP/rVSV at MOI = 0.2. Infection was assessed usi.....
Document: ISG-RFP lentiviral constructs were a kind gift from Dr. Charles Rice (Rockerfeller University) and have been previously described [40] . HEK 293T cells stably expressing TIM-1 (H3 cells) [23] , which are routinely tested for the absence of mycoplasma, were transfected with the ISG-RFP constructs at the indicated concentrations. Forty-eight hours following transfection, cells were infected with EBOV-GP/rVSV at MOI = 0.2. Infection was assessed using flow cytometry (BD FACSVerse) 24 hours following infection. Flow cytometry data were analyzed using FlowJo cytometry analysis software for percentage of RFP positive cells that were also GFP positive. Relative infection was determined based on infection of cells expressing RFPcontrol construct alone.
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