Author: Kumar, Satyendra; Arankalle, Vidya A.
Title: Intracranial Administration of P Gene siRNA Protects Mice from Lethal Chandipura Virus Encephalitis Document date: 2010_1_7
ID: 1mzq227n_26
Snippet: Mouse tissue preparation for real time one step RT-PCR, gene expression and histopathology Mice (n = 3) were euthanized and brains/spleens were removed and used in various experiments. For histopathology, the brain samples were fixed in 10% neutral buffered formalin, embedded in paraffin and 6-mm vertical sections were stained with hematoxylin and eosin. For the quantitative assessment of CHPV replication in mice brain using real time one step RT.....
Document: Mouse tissue preparation for real time one step RT-PCR, gene expression and histopathology Mice (n = 3) were euthanized and brains/spleens were removed and used in various experiments. For histopathology, the brain samples were fixed in 10% neutral buffered formalin, embedded in paraffin and 6-mm vertical sections were stained with hematoxylin and eosin. For the quantitative assessment of CHPV replication in mice brain using real time one step RT-PCR, RNA isolated using Ribopure RNA isolation Kit (Ambion, USA) according to manufacturer's instructions and 500 ng of total RNA was used per reaction [24] .
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