Author: Kumar, Satyendra; Arankalle, Vidya A.
Title: Intracranial Administration of P Gene siRNA Protects Mice from Lethal Chandipura Virus Encephalitis Document date: 2010_1_7
ID: 1mzq227n_26
Snippet: Mouse tissue preparation for real time one step RT-PCR, gene expression and histopathology Mice (n = 3) were euthanized and brains/spleens were removed and used in various experiments. For histopathology, the brain samples were fixed in 10% neutral buffered formalin, embedded in paraffin and 6-mm vertical sections were stained with hematoxylin and eosin. For the quantitative assessment of CHPV replication in mice brain using real time one step RT.....
Document: Mouse tissue preparation for real time one step RT-PCR, gene expression and histopathology Mice (n = 3) were euthanized and brains/spleens were removed and used in various experiments. For histopathology, the brain samples were fixed in 10% neutral buffered formalin, embedded in paraffin and 6-mm vertical sections were stained with hematoxylin and eosin. For the quantitative assessment of CHPV replication in mice brain using real time one step RT-PCR, RNA isolated using Ribopure RNA isolation Kit (Ambion, USA) according to manufacturer's instructions and 500 ng of total RNA was used per reaction [24] .
Search related documents:
Co phrase search for related documents- isolation kit and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- isolation kit and total RNA ng: 1, 2
- manufacturer instruction and real time: 1
- mice brain and Mouse tissue: 1, 2
- mice brain and quantitative assessment: 1
- mice brain and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9
- mice brain and RT PCR step: 1, 2
- mice brain and RT PCR step real time: 1, 2
- quantitative assessment and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
- real time and RT PCR step: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77
- real time and RT PCR step real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75
- real time and total RNA ng: 1, 2, 3, 4, 5, 6, 7
- real time and vertical section: 1
- RT PCR step and total RNA ng: 1, 2
Co phrase search for related documents, hyperlinks ordered by date