Author: Singh, Manmeet; Khan, Reas S.; Dine, Kimberly; Das Sarma, Jayasri; Shindler, Kenneth S.
Title: Intracranial Inoculation Is More Potent Than Intranasal Inoculation for Inducing Optic Neuritis in the Mouse Hepatitis Virus-Induced Model of Multiple Sclerosis Document date: 2018_9_4
ID: 03c9rx3o_17
Snippet: Serial sections from eye, optic nerve, and brain were stained by the avidin-biotin-immunoperoxidase technique (Vector Laboratories) using 3, 3-diaminobenzidine as substrate, and a 1:100 dilution of anti-Iba1 (Wako, Richmond, VA, USA), and 1:40 dilution of antiviral nucleocapsid antiserum (mouse monoclonal anti-N; Nucleocapsid protein of MHV-JHM, monoclonal clone 1-16-1, Kindly provided by Julian Lebowitz, Texas A&M, College Station, TX) as primar.....
Document: Serial sections from eye, optic nerve, and brain were stained by the avidin-biotin-immunoperoxidase technique (Vector Laboratories) using 3, 3-diaminobenzidine as substrate, and a 1:100 dilution of anti-Iba1 (Wako, Richmond, VA, USA), and 1:40 dilution of antiviral nucleocapsid antiserum (mouse monoclonal anti-N; Nucleocapsid protein of MHV-JHM, monoclonal clone 1-16-1, Kindly provided by Julian Lebowitz, Texas A&M, College Station, TX) as primary antibodies. Control slides from mock-infected mice were incubated in parallel.
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